2San Vitamin D Test

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Over 40% of adults are vitamin D deficient. Checking the levels of vitamin D in your blood to see if your levels are too low is essential for good health. Low vitamin D can cause symptoms such as low mood, fatigue and frequent illness. 

Vitamin D is a vital nutrient that plays an essential role in maintaining the immune system, bone strength, mood and energy levels. Vitamin D is found in some foods, but our main source is sunlight. During the winter months the sun’s rays may be weaker and vitamin D deficiency may be more common. 

Iron and good thyroid function also play a role in energy levels: 2San recommends these tests alongside the vitamin D test. 

All orders are sold at the professional discretion of our Well pharmacists, they may contact you if they require any further information before your order is dispatched.

How to use

  1. Pull off and dispose cap of lancet
  2. Clean fingertip with alcohol pad
  3. Press the lancet against the fingertip
  4. Massage the pricked fingertip to obtain blood drop
  5. Put capillary dropper in contact with blood and draw the blood to fill line
  6. Put the collected blood into the sample well
  7. Add 2 drops of the buffer 
  8. Wait for the coloured line and read results at 10 minutes – compare it to vitamin D Colour card

Full product preparation and usage is included in product box on IFU (instructions for use) 


What is the Vitamin D Rapid Test Cassette used for? 

The Vitamin D Rapid Test Cassette is a test used to detect the level of 25-hydroxyvitamin D (25 (OH) D) in human blood. It can help screen for Vitamin D deficiency. 

How does Vitamin D deficiency affect the body? 

Vitamin D deficiency can have severe health risks and is associated with diseases such as:  

  • Osteoporosis  
  • Multiple sclerosis 
  • Cardiovascular diseases 
  • Diabetes 
  • Depression 
  • and various infections. 

How does the Vitamin D test work? 

The test is based on competitive binding. The 25 (OH) D present in the blood specimen competes with the 25 (OH) D antigens on the test line for a limited amount of anti-25 OH vitamin D antibodies. The intensity of the test line determines the vitamin D level. 

How should I perform the test? 

Wash your hands, open the package, and place the test cassette on a clean surface. Use the lancet to puncture your finger, and collect blood with the capillary dropper. Add the blood to the specimen well and wait for the coloured lines to appear. Compare the test line intensity with the provided colour card. 

How should I interpret the test results? 

A deficient result means the Vitamin D level is less than 10ng/ml, an insufficient result means it is between 10ng/ml and 30ng/ml, and a sufficient result means it is equal to or higher than 30ng/ml. Refer to the colour card for comparison.   

Can the test results be incorrect? 

The results are accurate if the instructions are followed carefully. However, errors can occur if the test cassette gets wet, not enough blood is used, or the procedure isn’t followed correctly. False results are rare but can happen. Consulting a doctor is recommended for further guidance. 

When should I read the test results? 

The results should be read at 10 minutes after adding the buffer. Reading the results after 20 minutes may lead to unreliable results. 

What should I do if the result is deficient or insufficient? 

If the result indicates Vitamin D deficiency or insufficiency, consult a doctor to show them the test result. The doctor will determine if you need more tests or to take vitamin D supplements. 

What should I do if the result is sufficient? 

If the result is sufficient, it means the Vitamin D level is within the normal range. However, if symptoms persist, consult a doctor for further advice. 

How should I store the test kit? 

Store the kit in a dry place at temperatures between 2-30°C (36-86°F). Do not use the kit if the package is damaged. Keep it out of the reach of children.   

Can I reuse the test kit? 

No, the test kit is designed for single use only. Do not dismantle or touch the test window of the test cassette.   

Where can I get more information or help regarding the Vitamin D Test?  

 For more information, you may check the Instructions For Use or you can contact 2San at their address: 6-8 Greencoat Place, London SW1P 1PL, United Kingdom, or via phone: +44 1524 488 064, or email: helpdesk@2san.com. They also have a website www.2san.com